Methodology article
An efficient RNA interference screening strategy for gene functional analysis
1 Department of Computer Science, National Tsing Hua University, Hsinchu, 30013, Taiwan
2 Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, 10617, Taiwan
3 Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan
4 Orchid Research Center, National Cheng Kung University, Tainan, 701, Taiwan
5 Institute of Tropical Plant Scicenes, National Cheng Kung University, Tainan, 701, Taiwan
6 Research Center for Plant Medicine, National Taiwan University, Taipei, 10617, Taiwan
7 Department of Computer Science and Information Engineering, Providence University, Taichung, 43301, Taiwan
BMC Genomics 2012, 13:491 doi:10.1186/1471-2164-13-491
Published: 18 September 2012Additional files
Additional file 1:
Figures S1 and S2. A simple example of a powerful subsequence P, and the dotplot of TF15 and TF21 sequences.
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Additional file 2:
Proof to prove that the problem of finding a qualified sequence r maximizing the size of T is NP-complete.
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Additional file 3:
Methods the detail of our modified greedy algorithm for selecting qualified siRNAs to perform the first-round RNAi analysis.
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Additional file 4:
Tables S1-3. The lists of the siRNAs that were selected for first-round RNAi analysis with different parameters.
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