Additional file 5.
Figure S1. Dosage effect of sall genes. (A) mRNA-Seq expression plot for arm, Fas-3, Nrg, Fas2, CadN and N genes in heterozygous (He) and homozygous (Ho) sall mutant embryos at stage 16 showed consistent results with the differences in protein levels observed by Cantera et al. (Cantera et al. 2002). Notice that heterozygous sall mutant embryos have higher transcript levels than homozygous for all these adhesion and cytoskeleton genes, suggesting a dosage effect of Sall. (B) mRNA-Seq analysis of the transcriptome of WT16, He16 and Ho16 embryos, showed that five genes are differentially expressed (p < 0.01) between all the genotypes compared at stage 16 and have intermediate levels of expression in He16. (C) At stage 17, instead, four of the genes that are differentially expressed (p < 0.01) between all the genotypes compared at this stage had intermediate levels of expression in He17.
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Ferreiro et al. BMC Genomics 2012 13:483 doi:10.1186/1471-2164-13-483