The effect of threshold selection on the number and uniqueness of HECS genes. The distributions of the number of HECS genes per cell type as the threshold criteria used to define a HECS gene is raised from 2x to 25x the median expression value across all cell types for the (A) mouse and (B) human gene expression data. To quantify uniqueness, we determined the percentage of HECS genes that were mapped to n or fewer cell types (i.e., the cumulative %) for the (C) mouse and (D) human gene expression data for different threshold values. The results corresponding to the threshold values selected in the current implementation of CTen are colored blue and orange for the mouse and human data, respectively.
Shoemaker et al. BMC Genomics 2012 13:460 doi:10.1186/1471-2164-13-460