Additional file 10.

Conservation of miRNAs found in this article. In column 2–5 the conservation is based on full genomic pairwise alignments of pig and 17 other mammalian genomes (bosTau4, turTru1, equCab2, felCat3, canFam2, eriEur1, hg19, tarSyr1, mm9, rn4, oryCun2, loxAfr3, echTel1, dasNov2, choHof1, monDom5, ornAna1). The miRNA pig coordinates are required to transfer to the target organism, and the coordinates in the target organism is required to transfer back to the same position in pig to avoid paralogous alignments. The pig sequence is then aligned to the target sequence and a minimum align length of 50 and an identity of at least 50% is required for the miRNA to be counted in the third column. In columns 2 and 3 the pairwise identity between the pig sequence and cow or human is given, and in column 4 the number of organisms where the miRNA is found is given and finally in column 5 the mean pairwise identity is given. Columns 6–9 are analogous to column 2–5, except the identification of the miRNAs in the target organism are now identified by a BLAST of mirBase version 18 against the target genome. The pig and target sequence are then aligned in the same way as before.

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Podolska et al. BMC Genomics 2012 13:459   doi:10.1186/1471-2164-13-459