Table 4

Expression values of genes encoding enzymes in ubiquitin-proteasome pathway at 15, 90 and 250 days of lactation

Protein

Gene symbol1

Day 152

Day 902

Day 2502


UB activating enzyme (E1)

UBA1

57.96

128.64

134.10


UB-conjugating enzyme (E2)

CCDC34

0.32

1.14

1.47

BRCA1

0.48

1.87

2.71

UBE4B

5.83

9.83

13.01

UBE4A

8.19

23.34

17.83

UBE2R2

10.91

21.70

26.34

UFD1L

27.59

45.93

39.23

UBE2I

14.54

37.27

42.38

UBE2C

6.43

19.54

42.98

UBE2B

16.53

48.69

43.34


UB-protein ligase (E3)

SKP2

0.24

1.00

1.07

CUL5

0.93

1.80

2.39

ANAPC10

1.63

3.12

3.05

SMURF2

1.48

4.72

4.45

CUL4B

3.02

4.87

5.29

CUL2

1.62

6.14

5.96

BTRC

2.11

12.92

6.21

SMAD3

3.03

10.07

6.36

ANAPC5

3.14

7.08

6.94

ANAPC1

1.73

8.01

8.20

CUL3

3.62

8.21

8.59

CUL1

4.88

14.32

14.64

FBXW2

9.39

27.72

25.13

SMAD2

5.46

20.50

26.03

CUL4A

15.25

59.93

44.91

SKP1

14.72

61.88

48.03

STUB1

42.41

55.86

52.47

RBX1

33.24

42.01

76.38


UB and UB-like proteins

SUMO1

0.88

0.70

1.15

UBL7

49.97

18.95

22.41

UBAP1

8.78

32.96

28.78

NEDD8

50.89

98.49

106.18

UBD

32.48

163.89

259.41

UBC

112.33

1095.10

332.69

RPS27A

551.89

451.18

630.29

UBA52

1710.22

1277.97

1903.08


Deubiquitination enzymes

USP2

0.60

0.37

0.23

USP9X

0.05

0.46

0.56

USP21

2.65

5.23

6.28

VCPIP1

4.84

18.33

12.68

USP8

4.54

10.15

14.05

USP10

7.26

17.51

23.79


Proteasome

ATAD2

0.66

3.37

2.39

ATAD3A

8.23

13.67

11.38

ATAD1

4.43

9.99

13.15

PSMA1

18.19

49.89

69.91

PSMD2

41.15

82.81

112.20

PSMB2

39.62

84.58

115.62


1Gene symbol according to NCBI Entrez gene database http://www.ncbi.nlm.nih.gov/gene/ webcite

2Days 15, 90 and 250 gene expressions given in RPKM

Wickramasinghe et al. BMC Genomics 2012 13:45   doi:10.1186/1471-2164-13-45

Open Data