Analytical flowchart to study bovine milk transcriptome by RNA-Seq. Nine milk samples was collected at 15, 90 and 250 days of lactation with three biological replicates per stage. An initial global analysis was conducted on genes expressed in bovine MSC followed by a detailed analysis on genes encoding enzymes in major milk component synthesis pathways, endogenous milk proteases and enzymes in ubiqutin-proteasome pathway.
Wickramasinghe et al. BMC Genomics 2012 13:45 doi:10.1186/1471-2164-13-45