Table 2

Plasmids and bacteria used in this work
Plasmids Relevant genotype/information Source/reference
pK18mobsacB sacB, lacZα, mcs (KmR) [49]
pET2 E. coliC. glutamicum promoter-probe vector (KmR, promoterless cat gene) [58]
pET2sigH sigH promoter region (550 bp) in pET2 this work
pET2rshA rshA promoter region (301) in pET2 this work
pET2sigH4 P4sigH promoter region (348 bp) in pET2 this work
E. coli
E. coli JM109 endA1, recA1, gyrA96, thi, hsdR17 (rk, mk+), relA1, supE44, Δ(lac-proAB), F´ traD36, proAB, laqIqlacZΔM15 [59]
C. glutamicum
RES167 restriction-deficient C. glutamicum strain ( ΔcglIM-cglIR-cglIIR) [47]
DN2 RES167 deletion of sigH [24]
AS1 RES167 deletion of sigH-rshA this work
ΔrshA RES167 deletion of rshA this work

Busche et al.

Busche et al. BMC Genomics 2012 13:445   doi:10.1186/1471-2164-13-445

Open Data