Coverage characteristics for the sequenced genome. The coverage calculations ignore potential PCR duplicates and secondary alignments. (A) Coverage depth distribution of the sequenced genome. The y-axis indicates the fraction of the non-N bases in the reference sequence that has a particular coverage on the x-axis. The curve in gray shows the Poisson distribution with the best fit to the distribution. (B) GC characteristics of the aligned data. We calculate and plot the GC percentage and average depth in non-overlapping windows of 50 kbp.
Gupta et al. BMC Genomics 2012 13:440 doi:10.1186/1471-2164-13-440