Table 3

Comparison of the current methods versus PBS.

Samples number

PSS 1

SBS 2

Combination of PSS 1 and SBS 2

PBS


10

Allowing up to 8 samples

10 barcoded oligos

4 segments with 3 barcoded oligos

7 barcoded oligos

(4 F-barcodes and 3 R-barcodes)

100

N/A

100 barcoded oligos

8 segments with 13 barcoded oligos

20 barcoded oligos

(10 F-barcodes and 10 R-barcodes)

1000

N/A

1000 barcoded oligos

8 segments with 125 barcoded oligos

67 barcoded oligos

(34 F-barcodes and 33 R-barcodes)

10000

N/A

10000 barcoded oligos

8 segments with 1250 barcoded oligos

200 barcoded oligos

(100 F-barcodes and 100 R-barcodes)


1 PSS, Physical Space segregation

2 SBS, Single barcode sequencing

Tu et al. BMC Genomics 2012 13:43   doi:10.1186/1471-2164-13-43

Open Data