Open Access Highly Accessed Methodology article

Pair-barcode high-throughput sequencing for large-scale multiplexed sample analysis

Jing Tu1, Qinyu Ge2, Shengqin Wang1, Lei Wang1, Beili Sun1, Qi Yang1, Yunfei Bai1 and Zuhong Lu12*

Author Affiliations

1 State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China

2 Key laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China

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BMC Genomics 2012, 13:43  doi:10.1186/1471-2164-13-43

Published: 25 January 2012

Additional files

Additional file 1:

Statistics after mapping. Statistics after mapping decoded NGS reads to the other non coding RNAs, Human Genome (RefSeq Hg19) and miRBase (Release 14.0).

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Additional file 2:

MiRNA read length distribution. Exhibit the miRNA read length distribution of each patient.

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Additional file 3:

MiRNA read counts of two independent runs. Scatter plots of miRNA expressions of all 32 barcode pairs of two independent runs.

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Additional file 4:

MiRNA read counts of different barcode pairs. Scatter plots of miRNA read counts of the same sample without barcode to with different barcode pairs.

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Additional file 5:

Highly expressed miRNAs. Highly expressed miRNAs in all 25 datasets.

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Additional file 6:

Mapping and normalization. The detailed progression of mapping and normalization.

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Additional file 7:

Different expressed miRNAs. The 22 miRNAs which were differentially expressed between breast malignant and breast carcinoid based on a t-test.

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Additional file 8:

Clinical data. Clinical data of the patients included in this study.

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Additional file 9:

Encoding and decoding. Details of the encoding and decoding progress.

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