Table 5

Genes with significant expression differences in the R group vs. S group in qRT-PCR.P< 0.05, Fold difference > 1.5
GlymaID PFAMa GO functionb PANTHERc KOGd BLASTP E-value BLAST hit species Contrasts Sampling points (hai)
12 24 48 72_Ih 72_Ui
Glyma19g35340 Zinc-binding dehydrogenase Zinc ion binding Alcohol dehydrogenase related Alcohol dehydrogenase, class III Alcohol dehydrogenase, putative 0E + 00 Ricinus communis CCe
RCf 1.5
ICg
Glyma19g40800 WD domain, G-beta repeat - WD repeat protein WD-repeat protein WDR6, WD repeat superfamily Transducin/WD40 domain-containing protein 0E + 00 Arabidopsis thaliana CC
RC 1.8
IC
Glyma19g40940 Glycosyl hydrolases family 28 Carbohydrate metabolism; polygalacturonase activity - - Glycoside hydrolase family 28 protein 0E + 00 Arabidopsis thaliana CC
RC −1.9
IC −1.6
Glyma19g40950 WRKY DNA -binding domain Transcription factor activity; sequence-specific DNA binding - - Putative WRKY transcription factor 42 9E-91 Arabidopsis thaliana CC
RC
IC −1.5
Glyma19g40970 AUX/IAA family Transcription factor activity - - Auxin-responsive protein IAA20, putative 1E-48 Ricinus communis CC −1.5
RC −1.9
IC −2.2 −1.8
Glyma19g41580 - - - - Transcription factor bHLH149 1E-30 Arabidopsis thaliana CC −1.6 −2.8
RC 1.5 2.3
IC
Glyma19g41780 GATA zinc finger Transcription factor activity; regulation of transcription, DNA-dependent; zinc ion binding; sequence-specific DNA binding Transcription factor gata (gata binding factor) - GATA transcription factor 16 1E-23 Arabidopsis thaliana CC
RC −1.5
IC
Glyma19g41800 Kinesin motor domain ATPase activity microtubule binding microtubule motor activity Kinesin heavy chain Kinesin (KAR3 subfamily) Kinesin heavy chain, putative 0E + 00 Ricinus communis CC −5.1 −5.6 −6.7 −2.9 −3.3
RC 2.0
IC −5.4 −6.2 −7.1 −6.2 −2.7
Glyma19g41870 Protein phosphatase 2C Protein serine/threonine phosphatase activity Protein phosphatase 2c Serine/threonine protein phosphatase Protein phosphatase 2c, putative 0E + 00 Ricinus communis CC
RC 1.6
IC
Glyma19g41900 F-box domain - - - Phloem-specific lectin PP2-like protein 2E-82 Arabidopsis thaliana CC
RC 1.9
IC
Glyma19g41930 Leucine Rich Repeat Protein binding F-box/leucine rich repeat protein Leucine rich repeat proteins, some proteins contain F-box Ubiquitin-protein ligase, putative 0E + 00 Ricinus communis CC
RC 2.1
IC
Glyma19g42050 Calcineurin-like phosphoesterase Protein serine/threonine phosphatase activity Serine/threonine protein phosphatase Serine/threonine specific protein phosphatase PP1, catalytic subunit Serine/threonine-protein phosphatase PP1 isozyme 8 0E + 00 Arabidopsis thaliana CC
RC 1.8
IC
Glyma19g42120 - - - Uncharacterized conserved protein heparan-alpha-glucosaminide N-acetyltransferase 0 + 00 Arabidopsis thaliana CC
RC 2.0
IC
Glyma19g42200 Rapid ALkalinization Factor (RALF) Signal transducer activity - - Rapid ALkalinization Factor 9e-47 Medicago truncatula CC
RC 1.6
IC
Glyma19g42340 Protein tyrosine kinase Protein-tyrosine kinase activity; protein amino acid phosphorylation; ATP binding Mapkk-related serine/threonine protein kinases MEKK and related serine/threonine protein kinases Mitogen activated protein kinase kinase kinase 3, mapkkk3, mekk3, putative 0E + 00 Ricinus communis CC
RC 2.8
IC 2.1 2.0

R group: lines with the Conrad haplotype (Conrad, the RILs 2022, and 1960); S group: lines with the Sloan haplotype (Sloan, the RILs 1974, and 2022). Empty cell indicates that there was no significant difference, and a negative value indicates the fold of greater expression ratio in the group with the Sloan haplotype.

a PFAM description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php webcite, updated on July 2011);

b Gene Ontology Descriptions obtained from the (http://soybase.org webcite, updated on July 2011);

c PANTHER description provided by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php webcite, updated on July 2011);

d KOG Description assigned by the Soybean Genome Project, DoE Joint Genome Institute (http://www.phytozome.net/soybean.php webcite, updated on July 2011);

e CC: Constitutive contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in mock-inoculated samples at specified time points (hai);

f RC: Response contrast—significant fold differences in infection response ratios (inoculated / mock-inoculated) in the group with Conrad haplotype compared to the group with Sloan haplotype at specified time points (hai);

g IC: Infection contrast—significant fold difference in transcript abundance between the group with Conrad haplotype and the group with Sloan haplotype in infected samples at specified time points (hai);

h Samples were collected from the inoculation site at 72 HAI;

i Samples were collected from the front of lesion margin at 72 HAI.

Wang et al.

Wang et al. BMC Genomics 2012 13:428   doi:10.1186/1471-2164-13-428

Open Data