Table 3

Identification of genes putatively involved in the biosynthesis of various vitamins and siroheme
Function Locus tag (Protein) Annotation Closest homolog outsideFrancisella locus tag, protein, identity,E-value
Thiamine biosynthesis
Fphi_0086 (251 aa) Thiazole biosynthesis adenylyltransferase (ThiF) Mrub_1727, 266 aa, 38%, 7e-41
Fphi_0087 (485 aa) Fused protein Phosphomethylpyrimidine kinase (ThiD)/Thiamine-phosphate pyrophosphorylase (ThiE) lpg1568, 495 aa, 35%, 1e-72
Fphi_0088 (259 aa) Thiazole synthase (ThiG) lpg1567, 263 aa, 58%, 2e-86
Fphi_1923 (66 aa) Thiamine biosynthesis protein (ThiS) IL0768, 66 aa, 32%, 1e-04
Fphi_0089 (350 aa) Thiamine biosynthesis oxidoreductase (ThiO) Kkor_0127, 351 aa, 34%, 3e-47
Fphi_0090 (592 aa) Thiamine biosynthesis protein (ThiC) CV_0235, 632 aa, 72%, 0.0
Riboflavin biosynthesis
Fphi_0395 (306 aa) Riboflavin kinase (RibF) D11S_1924, 308 aa, 47%, 5e-69
Fphi_0713 (356 aa) Riboflavin biosynthesis protein (RibD) Acear_1431, 371 aa, 44%, 5e-79
Fphi_0714 (201 aa) Riboflavin synthase, alpha subunit (RibC/RibE) CLD_1677, 228 aa, 48%, 5e-41
Fphi_0715 (403 aa) GTP cyclohydrolase II (RibA) DEFDS_1098, 405 aa, 49%, 5e-104
Fphi_0716 (147 aa) 6,7-dimethyl-8-ribityllumazine synthase (RibH) lpg1180, 155 aa, 56%, 3e-39
Folate biosynthesis
Fphi_0420 (282 aa) Methenyltetrahydrofolate cyclohydrolase (FolD) SULAZ_0428, 284 aa, 60%, 3e-87
Fphi_0547 (394 aa) Dihydrofolate synthase (FolC) Ssed_1653, 421 aa, 34%, 2e-45
Fphi_0600 (165 aa) Dihydrofolate reductase (FolA) BMD_4044, 161 aa, 54%, 7e-41
Fphi_1791 (184 aa) Para-aminobenzoate synthase, amidotransferase component Slin_0655, 205 aa, 38%, 4e-37
Fphi_1792 (587 aa) Para-aminobenzoate synthase, aminase component Csal_2692, 629 aa, 37%, 3e-99
Fphi_1794 (117 aa) Dihydroneopterin aldolase (FolB) WPa_0696, 125 aa, 32%, 1e-09
Fphi_1795 (421 aa) Dihydropteroate synthase (FolKP) RBE_0032, 438 aa, 35%, 4e-48
Biotin biosynthesis
Fphi_1798 (428 aa) Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (BioA) CBU_1008, 442 aa, 51%, 4e-121
Fphi_1799 (313 aa) Biotin synthase (BioB) CBU_1007, 321 aa, 61%, 7e-103
Fphi_1800 (372 aa) 8-amino-7-oxononanoate synthase (BioF) Sde_3138, 397 aa, 38%, 6e-60
Fphi_1801 (244 aa) Biotin biosynthesis protein (BioC) CBU_1004, 248 aa, 28%, 2e-10
Fphi_1803 (226 aa) Dethiobiotin synthase (BioD) Sde_3135, 230 aa, 38%, 5e-37
Siroheme biosynthesis
Fphi_0284 (323 aa) Porphobilinogen synthase (HemB) Amet_0062, 324 aa, 60%, 1e-110
Fphi_0603 (469 aa) Glutamyl-tRNA synthetase (GltX) Fbal_2571, 470 aa, 60%, 2e-163
Fphi_0691 (300 aa) Porphobilinogen deaminase (HemC) APL_1010, 309 aa, 56%, 4e-86
Fphi_0945 (344 aa) Uroporphyrinogen decarboxylase (HemE) Rmag_1026, 347 aa, 50%, 1e-97
Fphi_1071 (414 aa) Glutamyl-tRNA reductase (HemA) PSPA7_5315, 422 aa, 35%, 2e-70
Fphi_1313 (252 aa) Uroporphyrinogen-III synthase (HemD) PSM_A0104, 613 aa, 31%, 1e-08
Fphi_1400 (400 aa) Protoporphyrinogen oxidase (HemY/HemG) Cphamn1_2137, 396 aa, 34%, 2e-58
Fphi_1812 (433 aa) Glutamate-1-semialdehyde aminotransferase (HemL) APJL_1583, 426 aa, 61%, 2e-151
Fphi_1842 (308 aa) Coproporphyrinogen III oxidase (HemF) Tcr_0017, 323 aa, 59%, 2e-106
Fphi_1900 (338 aa) Ferrochelatase, protoheme ferro-lyase (HemH) SMc04019, 342 aa, 50%, 7e-92

Siddaramappa et al.

Siddaramappa et al. BMC Genomics 2012 13:422   doi:10.1186/1471-2164-13-422

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