Open Access Research article

Expression of cassini, a murine gamma-satellite sequence conserved in evolution, is regulated in normal and malignant hematopoietic cells

Anna Arutyunyan1, Sonia Stoddart1, Sun-ju Yi1, Fei Fei1, Min Lim1, Paula Groffen2, Niklas Feldhahn13, John Groffen145 and Nora Heisterkamp145*

Author Affiliations

1 Section of Molecular Carcinogenesis, Division of Hematology/Oncology and The Saban Research Institute of Children’s Hospital Los Angeles, Los Angeles, CA, 90027, USA

2 Medical Oncology, University Medical Center Groningen, Hanzeplein 1, 9713, GZ, Groningen, The Netherlands

3 Laboratory of Molecular Immunology, The Rockefeller University, New York, NY, 10065, USA

4 Leukemia Research Program, Children’s Hospital Los Angeles, Los Angeles, CA, USA

5 Leukemia and Lymphoma Program, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA

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BMC Genomics 2012, 13:418  doi:10.1186/1471-2164-13-418

Published: 23 August 2012

Additional files

Additional file 1:

Figure S1.A cluster of genes near the telomere of mouse chromosome 9. Graphic respesentation of Affymetrix microarray results and table describing 40 kb region on mouse chromosome 9 band A1.

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Additional file 2:

Figure S2.Deduced amino acid sequences of the cluster of Genbank-designated genes on mouse chromosome 9. Alignment of deduced amino acid sequences of chromosome 9 cluster.

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Additional file 3:

Figure S3.Translation of different physical mouse cDNAs including AK089719 (cassini) with substantial homology to the mouse chromosome 9 cluster. The deduced amino acid sequence of mouse cDNAs are aligned.

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Additional file 4:

Figure S4.Cassini protein. Translation of cassini cDNA, recurring amino acid motifs, and predicted secondary structure showing putative transmembrane regions.

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Additional file 5:

Figure S5.Protein product of EGFP-tagged cassini cDNA. Detection of EGFP-tagged Cassini protein in transfected COS-1 cells using polyclonal antisera and EGFP fluorescence.

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Additional file 6:

Description of data, methods and references for Figures S1-S6. Contains a description of the results shown in Figures S1-S6, the methods that were used to generate those data, and references relevant to this information.

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Additional file 7:

Figure S6.Effect of cassini on survival of drug-treated cells. 293 HEK cells were transfected with control pEGFP-C1 or pEGFP-cassini and compared for viability and cell numbers after treatment for 24 hours with the chemotherapeutic drugs cisplatin, etoposide and doxorubicin.

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