Table 6

Validation of microarray results and expression of genes of interest in relation to the factor 'Lipid level'
Gene Low n-3 LC-PUFA HL/LL High n-3 LC-PUFA HH/LH
Microarray RT-qPCR Microarray RT-qPCR
LC-PUFA biosynthesis
Δ5fad −1.03 1.35
Δ6fad 1.04 −1.32
elovl2 −1.28 to −1.41 −1.51 −1.82 to −1.97 −1.62
Cholesterol biosynthesis
ipi −3.95 1.31
mev −1.40 1.14
7dchr −1.01 1.09
srebp2 −2.09 1.29
Lipid transport and lipoprotein metabolism
lrp1 −1.82 −1.08
apoA4a −1.37 −0.95
apoA4b −2.43 −1.80
lpl −1.18 −1.05
Immune response
cfh −1.33 to −1.56 −1.24 −1.56 to −1.69 −1.31
trim25 −5.64 −2.09 −4.34 −2.33
lect2 3.16 1.23 4.86 4.84

Values represent the expression ratios between high lipid / low lipid, for fish containing either low or high n-3 LC-PUFA levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.

Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); complement factor H precursor (cfh); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).

Morais et al.

Morais et al. BMC Genomics 2012 13:410   doi:10.1186/1471-2164-13-410

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