Table 5

Validation of microarray results and expression of genes of interest in relation to the factor 'n-3 LC-PUFA level'
Gene Low Lipid LH/LL High Lipid HH/HL
Microarray RT-qPCR Microarray RT-qPCR
LC-PUFA biosynthesis
Δ5fad −1.19 1.17
Δ6fad 1.13 −1.21
elovl2 1.14 1.06
Cholesterol biosynthesis
ipi −2.13 −3.92 −1.15 1.32
mev −1.54 −1.51 −1.08 1.06
7dchr −1.33 to −1.54 −1.47 −1.25 to −1.47 −1.34
srebp2 −1.72 −1.68 −1.30 1.60
Lipid transport and lipoprotein metabolism
lrp1 −1.14 to −3.57 −1.36 −1.39 to −1.67 1.24
apoA4a −1.43 −1.09 1.00 1.32
apoA4b −1.39 −1.48 −1.43 −1.10
lpl 1.31 1.23 1.31 1.38
Glycerophospholipid synthesis
lpp2 1.20 −1.19 1.19 1.30
mgat 1.20 1.04 1.51 1.78
Eicosanoid biosynthesis
alox5 1.91 1.48 1.55 1.62
pla2g4 1.26 −1.08 1.08 1.06
thas −1.33 −1.08 −1.67 1.34
ptgis −2.53 −1.25 −4.25 1.27
Immune response
mal −22.20 −3.70 −25.00 −5.00
ccl13 4.40 5.98 3.00 2.14
trim25 5.20 2.80 6.70 2.51
lect2 4.60 1.92 7.10 7.57

Values represent the expression ratios between high PUFA / low PUFA, for fish containing either low or high total lipid levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.

Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); lipid phosphate phosphohydrolase 2 (lpp2); monoacylglycerol O-acyltransferase 1 (mgat); arachidonate 5-lipoxygenase (alox5); phospholipase A2 (pla2g4); thromboxane-A synthase (thas); prostaglandin I2 (prostacyclin) synthase (ptgis); myelin and lymphocyte protein (mal); c-c motif chemokine 13 precursor (ccl13); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).

Morais et al.

Morais et al. BMC Genomics 2012 13:410   doi:10.1186/1471-2164-13-410

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