Table 5 |
||||
| Validation of microarray results and expression of genes of interest in relation to the factor 'n-3 LC-PUFA level' | ||||
| Gene | Low Lipid LH/LL | High Lipid HH/HL | ||
| Microarray | RT-qPCR | Microarray | RT-qPCR | |
| LC-PUFA biosynthesis | ||||
| Δ5fad | −1.19 | 1.17 | ||
| Δ6fad | 1.13 | −1.21 | ||
| elovl2 | 1.14 | 1.06 | ||
| Cholesterol biosynthesis | ||||
| ipi | −2.13 | −3.92 | −1.15 | 1.32 |
| mev | −1.54 | −1.51 | −1.08 | 1.06 |
| 7dchr | −1.33 to −1.54 | −1.47 | −1.25 to −1.47 | −1.34 |
| srebp2 | −1.72 | −1.68 | −1.30 | 1.60 |
| Lipid transport and lipoprotein metabolism | ||||
| lrp1 | −1.14 to −3.57 | −1.36 | −1.39 to −1.67 | 1.24 |
| apoA4a | −1.43 | −1.09 | 1.00 | 1.32 |
| apoA4b | −1.39 | −1.48 | −1.43 | −1.10 |
| lpl | 1.31 | 1.23 | 1.31 | 1.38 |
| Glycerophospholipid synthesis | ||||
| lpp2 | 1.20 | −1.19 | 1.19 | 1.30 |
| mgat | 1.20 | 1.04 | 1.51 | 1.78 |
| Eicosanoid biosynthesis | ||||
| alox5 | 1.91 | 1.48 | 1.55 | 1.62 |
| pla2g4 | 1.26 | −1.08 | 1.08 | 1.06 |
| thas | −1.33 | −1.08 | −1.67 | 1.34 |
| ptgis | −2.53 | −1.25 | −4.25 | 1.27 |
| Immune response | ||||
| mal | −22.20 | −3.70 | −25.00 | −5.00 |
| ccl13 | 4.40 | 5.98 | 3.00 | 2.14 |
| trim25 | 5.20 | 2.80 | 6.70 | 2.51 |
| lect2 | 4.60 | 1.92 | 7.10 | 7.57 |
Values represent the expression ratios between high PUFA / low PUFA, for fish containing either low or high total lipid levels in their flesh, obtained by microarray analysis or RT-qPCR. Expression ratios in bold were significant by REST2008 analysis of RT-qPCR results.
Delta5 and 6 fatty acyl desaturases (Δ5fad and Δ6fad); fatty acyl elongase (elovl2); isopentenyl-diphosphate isomerase (ipi); mevalonate kinase (mev); 7-dehydrocholesterol reductase (7dchr); sterol regulatory element-binding protein 2 (srebp2); low density lipoprotein receptor-related protein 1 (lrp1); apolipoprotein A-IV (apoa4a and apoa4b); lipoprotein lipase (lpl); lipid phosphate phosphohydrolase 2 (lpp2); monoacylglycerol O-acyltransferase 1 (mgat); arachidonate 5-lipoxygenase (alox5); phospholipase A2 (pla2g4); thromboxane-A synthase (thas); prostaglandin I2 (prostacyclin) synthase (ptgis); myelin and lymphocyte protein (mal); c-c motif chemokine 13 precursor (ccl13); tripartite motif-containing protein 25 (trim25); leukocyte cell-derived chemotaxin 2 precursor (lect2).
Morais et al. BMC Genomics 2012 13:410 doi:10.1186/1471-2164-13-410
