Table 4

Lipid metabolism genes differentially expressed in the liver of fish having high or low flesh n-3 LC-PUFA contents
Probe name Gene High/Low LC-PUFA p- value
L Lipid (LH/LL) H Lipid (HH/HL)
Cholesterol biosynthesis, metabolism and regulation
Omy#S15288895 Sterol-C4-methyl oxidase-like 1.84 1.32 0.0033
Ssa#STIR00031_3 7-dehydrocholesterol reductase (7dchr) - 1.55 - 1.48 0.0036
Ssa#S30286041 7-dehydrocholesterol reductase (7dchr) - 1.48 - 1.42 0.0120
Omy#CF752841 Sterol regulatory element-binding transcription factor 2 (srebp2) - 1.71 - 1.31 0.0170
Ssa#TC102141 Cytochrome P450, family 27, subfamily A, polypeptide 1 - 3.97 - 1.04 0.0192
Ssa#STIR16974 7-dehydrocholesterol reductase (7dchr) - 1.46 - 1.31 0.0195
Ssa#AM402497 Hydroxymethylglutaryl-CoA synthase 1 - 2.39 1.21 0.0199
Ssa#STIR00031_4 7-dehydrocholesterol reductase (7dchr) - 1.41 - 1.46 0.0212
Ssa#STIR00098_4 Isopentenyl-diphosphate delta isomerise (ipi) - 2.12 - 1.15 0.0228
Ssa#S18867829 7-dehydrocholesterol reductase (7dchr) - 1.33 - 1.25 0.0228
Ssa#DY741343 Lanosterol 14-alpha demethylase - 1.42 - 1.28 0.0346
Omy#S22913656 Acetoacetyl-CoA synthetase - 1.77 - 2.52 0.0358
Ssa#STIR00033_3 Mevalonate kinase (mev) - 1.54 - 1.08 0.0393
Ssa#CA064135 Vigilin 2.80 - 1.22 0.0423
Ssa#DW582478 Cytochrome P450, family 8, subfamily B, polypeptide 1 - 1.26 - 1.50 0.0449
Ssa#STIR00102_3 Squalene epoxidase - 2.11 - 1.36 0.0468
Glycerophospholipid synthesis
Ssa#STIR39152_S Lipid phosphate phosphohydrolase 2 (lpp2) 1.20 1.19 0.0177
Ssa#KSS4003 Chka protein 1.18 1.53 0.0264
Ssa#S35538062_S Monoacylglycerol O-acyltransferase 1 (mgat) 1.20 1.51 0.0303
Ssa#S31963704 Diacylglycerol O-acyltransferase homolog 2 1.41 1.02 0.0358
Ssa#S48418830 Phosphatidylglycerophosphate synthase 1 - 1.52 - 1.72 0.0369
Fatty acid synthesis
Ssa#KSS4155 Trans-2-enoyl-CoA reductase, mitochondrial precursor - 1.26 - 1.25 0.0440
Intracellular fatty acid transport
Ssa#S35585414 Acyl-CoA-binding protein 10.47 1.65 0.0081
Ssa#DY703528 Fatty acid-binding protein, intestinal - 1.25 - 1.46 0.0331
Ssa#CB509140 Fatty acid-binding protein, intestinal - 1.20 - 1.35 0.0375
Ssa#STIR04578 Fatty acid-binding protein, heart 1.22 1.47 0.0401
Lipid transport
Ssa#CK898816 Low density lipoprotein receptor-related protein 1 (lrp1) - 1.14 - 1.39 0.0341
Ssa#S32008850 Apolipoprotein A-IV precursor (apoa4a) - 1.43 - 1.01 0.0377
Omy#BX318293 Low density lipoprotein receptor-related protein 1 (lrp1) - 3.61 - 1.67 0.0404
Ssa#S18866963 Apolipoprotein A-IV precursor (apoa4b) - 1.39 - 1.43 0.0428
Lipid hydrolysis
Ssa#S18887340 Acyl-coenzyme A thioesterase 5 1.64 1.18 0.0007
Ssa#STIR02708 Isoamyl acetate-hydrolyzing esterase 1 homolog 1.54 1.12 0.0013
Ssa#DW007099 Similar to patatin-like phospholipase domain containing 7 - 3.75 - 2.37 0.0070
Ssa#S31963297 Acyl-CoA thioesterase 11 1.35 1.38 0.0097
Ssa#STIR07750 N-acylsphingosine amidohydrolase 1 - 1.14 - 1.34 0.0122
Ssa#STIR05034 Isoamyl acetate-hydrolyzing esterase 1 homolog 1.74 1.01 0.0208
Omy#CA366823 Acyl-coenzyme A thioesterase 3 1.33 2.17 0.0248
Ssa#STIR22551 Lipoprotein lipase (lpl) 1.31 1.31 0.0346
Eicosanoid synthesis and metabolism
Ssa#TC68569 Thromboxane-A synthase (thas) - 1.33 - 1.67 0.0269
Ssa#TC110080 Phospholipase A2 (pla2g4) 1.26 1.08 0.0296
Omy#TC147730 Prostaglandin I2 (prostacyclin) synthase (ptgis) - 2.53 - 4.25 0.0349
Ssa#S35581706 15-hydroxyprostaglandin dehydrogenase - 1.51 - 1.09 0.0400
Ssa#EG930234 Arachidonate 5-lipoxygenase (alox5) 1.91 1.55 0.0449
Regulation of lipid metabolism
Ssa#TC112002 Retinoid X receptor beta 1.00 - 19.09 0.0000
Ssa#KSS2129 Adiponectin receptor protein 1 - 1.30 - 1.31 0.0098
Ssa#CA056493 Angiopoietin-like 6 - 1.63 - 1.07 0.0290
Ssa#S35490606 Adiponectin, C1Q and collagen domain containing, like 1.34 1.66 0.0441
Ssa#S18888608 Adiponectin receptor protein 1 - 1.38 - 1.40 0.0451

Results were obtained by two-way ANOVA analysis (pā€‰<ā€‰0.05; fold change cut-off of 1.2) without multiple testing correction (nā€‰=ā€‰1951 total features). Transcripts are arranged by functional categories and, within these, by p-value. Indicated are also the probe names and the expression ratio between families determined separately for each total lipid level.

Morais et al.

Morais et al. BMC Genomics 2012 13:410   doi:10.1186/1471-2164-13-410

Open Data