Phylogenetic analysis of the amino acid sequences of the 53 TNLs. The evolutionary history was inferred using the Maximum Likelihood method based on the JTT matrix-based model. The bootstrap consensus tree inferred from 500 replicates is taken to represent the evolutionary history of the sequences analyzed. Branches corresponding to partitions reproduced in less than 50% of bootstrap replicates are collapsed. The two subfamilies, Rosoideae and Spiraeoideae, are clearly separated. The TNLs from the genera Rosa and Fragaria are also separate. Intermixed branching is shown for the 20 Rdr1-TNLs from R. multiflora and R. rugosa. The 23 TNLs of apple and peach are clustered in multiple clades or subclades. The TNLs for each clade or subclade are species specific, with few intermixing. The tree is drawn to scale, with branch lengths measured in terms of the number of substitutions per site. Bootstrap values of more than 50 are indicated next to the branches.
Terefe-Ayana et al. BMC Genomics 2012 13:409 doi:10.1186/1471-2164-13-409