Identification and characterization of RNase 8 transcript. (A) ~800 and ~1000 bp transcripts encoding RNase 8 were isolated from human spleen cDNA using a nested rapid amplification of cDNA ends (RACE); +, including adapter primer;-without adapter primer. (B) Findings from RACE confirmed by RT-PCR using two unique sources of poly A+ RNA; +, including reverse transcriptase;-without reverse transcriptase. (C) RNase 8 on human chromosome 14; black bar, coding sequence originally described  green bar, novel amino terminal extension. RACE amplification products are shown above extending 0.8 and 1 kb from primer within the RNase 8 coding sequence; nested RT-PCR amplification product (0.5 kb) is also as shown below. The GenBank accession number for the spleen cDNA RNase 8 transcript sequences is JQ353679.
Chan et al. BMC Genomics 2012 13:40 doi:10.1186/1471-2164-13-40