Table 1 |
||||
| Genes analyzed by quantitative real-time PCR | ||||
| Gene | Primer sequence | Size (bp) | Accession number (GenBank) | |
| Upregulated genes/sequences | APP | F: ACCCCTGACGCCGTTGAC | 121 | NM_001076796.1 |
| R: TCATGACCTGGGACATCCTCTC | ||||
| AQP4 | F: GTTCACGGAAATCTTAGCGCT | 104 | NM_181003.2 | |
| R: TCAGTCCGTTTGGAATCACAG | ||||
| CPPED1 | F: TTGGATGGCATCACCGACTT | 101 | NM_001031771.2 | |
| R: TTTGCGACCTCATGAACCAC | ||||
| GOLGA4 | F: TCTACCAAAACCACTGCCTCAA | 88 | NM_001192125.1 | |
| R: TCCCACTACTGGCTCTACATCACT | ||||
| MPP7 | F: GCCTCCTATGCCTGATGACAT | 81 | NM_001100347.1 | |
| R: CCCAGTGGTTCTCTATTTTTGACC | ||||
| NELL2 | F: AAGAGGGAGACGATGGACTGAG | 105 | NM_001102084.1 | |
| R: ACACCAAGACCCCAAACTGCT | ||||
| OSRS1* | F: GAGGATCTTGTGGAACCATTGA | 124 | FQ482089.2 | |
| R: TACGGACAGCTGAACCCTTTC | ||||
| OSRS2** | F: TTGTCAGTCCCCATCACCTTT | 101 | NW_003104406.1 | |
| R: CATTGATTTGCACAGAAAACCA | ||||
| Downregulated genes | CD3G | F: AGCTTCAGACAAGCAGACGCT | 101 | BC103010.1 |
| R: GGGTTCAGTTCTTCCTCAGGTG | ||||
| GNLY | F: TCCGTGCCAGTCAATCATGA | 101 | NM_001075143.1 | |
| R: TGCAGACCTTGATGTCCACAC | ||||
| LAPTM4B | F: GGTACTTGATCCTCAATGCCG | 101 | NM_205802.1 | |
| R: AAAGTCACCCCCGAGTTCAGA | ||||
| SRSF3 | F: CGAAATGCATCGTGATTCCT | 101 | NM_001034700.1 | |
| R: AATAGCCAAAAGCTCGTTCCA | ||||
Primers (F: forward and R: reverse) used for gene amplification, amplicon size and GenBank accession numbers of the bovine cDNA sequences used for primer design.
*Ovine Scrapie Related Sequence 1 (OSRS1) has a high identity (92%) with the Bos taurus DNA sequence from clone CH240-103 G5(accession number: FQ482089.2) from 4172 to 4904 bp.
**Ovine Scrapie Related Sequence 2 (OSRS2) has a high identity (93%) with the Bos taurus chromosome 15 genomic scaffold, Bos_taurus_UMD_3.1, whole genome shotgun sequence (accession number: NW_003104406.1) from 2672152 to 2672419 bp.
Filali et al. BMC Genomics 2012 13:399 doi:10.1186/1471-2164-13-399
