Open Access Research article

A physical map of a BAC clone contig covering the entire autosome insertion between ovine MHC Class IIa and IIb

Gang Li12, Ka Liu25, Shasha Jiao12, Haibo Liu2, Hugh T Blair34, Peng Zhang25, Xiaoran Cui2, Pingping Tan2, Jianfeng Gao14* and Runlin Z Ma2456*

Author Affiliations

1 School of Life Sciences, Shihezi University, Xinjiang 832003, China

2 State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China

3 Institute of Veterinary Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand

4 Joint Research Center for Sheep Breeding and Developmental Biology, IGDB-Massey University, Massey, New Zealand

5 Graduate University of Chinese Academy of Sciences, Beijing 100149, China

6 Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China

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BMC Genomics 2012, 13:398  doi:10.1186/1471-2164-13-398

Published: 16 August 2012

Additional files

Additional file 1:

Table S1. The ovine oligo primers used for verification of overlapping relationships of the positive BAC clones.

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Additional file 2:

Figure S1. A complete physical map of entire ovine MHC with the insertion region between class IIa and IIb included. Order and orientation of overlapping BAC clones were jointly determined by combinations of DNA fingerprinting, BAC-end sequencing, and sequence-specific PCR. Genes identified by BAC-end sequencing are marked with erect black lines, with their names listed above. A horizontal bar stands for individual BAC with its identification marked above. Red, purple and green color represent the MHC class I, class III, and class II, representatively.

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