Analysis of histone modifications at the promoter regions of M. domestica orthologues of eutherian imprinted genes.(A) Chromatin immunoprecipitation with H3K4me2 antibody followed by PCR amplification is shown for the IGF2R promoter region; L denotes the 100 bp ladder, I the input, R the sample immunoprecipitated with rabbit serum albumin (i.e. non-specific pull-down), and K4 the sample immunoprecipitated with H3K4me2 antibody. Analysis of enrichment was performed by real-time PCR following chromatin immunoprecipitation with H3K4me2 and H3K9me3 antibodies at the promoter regions of (B) IGF2R, (C) HTR2A, and (D) L3MBTL. The fold change is plotted on the Y-axis, and the distance from the putative transcription start site (0) on the X-axis. The black bars represent H3K4me2, and the grey bars represent H3K9me3.
Das et al. BMC Genomics 2012 13:394 doi:10.1186/1471-2164-13-394