Methylation profiles of M. domestica CpG-rich regions close to orthologues of eutherian imprinted genes. Bisulfite-modified genomic DNA was PCR amplified and cloned. Each line denotes an individual cloned allele. Representative CpG-rich regions analyzed are (A) putative promoter region of HTR2A, (B) 5′ upstream region of L3MBTL, (C) 5′ upstream region of MEST, (D) 5′ upstream region of IMPACT, (E) 5′ upstream region of COPG2, and (F) putative promoter region of IGF2R. Unfilled circles depict unmethylated cytosines at CpG sites, while filled circles depict methylated cytosines. The genomic co-ordinates of these regions are listed in Additional file 1: Table S3.
Das et al. BMC Genomics 2012 13:394 doi:10.1186/1471-2164-13-394