Figure 3.

Methylation profiles of M. domestica CpG-rich regions close to orthologues of eutherian imprinted genes. Bisulfite-modified genomic DNA was PCR amplified and cloned. Each line denotes an individual cloned allele. Representative CpG-rich regions analyzed are (A) putative promoter region of HTR2A, (B) 5 upstream region of L3MBTL, (C) 5 upstream region of MEST, (D) 5 upstream region of IMPACT, (E) 5 upstream region of COPG2, and (F) putative promoter region of IGF2R. Unfilled circles depict unmethylated cytosines at CpG sites, while filled circles depict methylated cytosines. The genomic co-ordinates of these regions are listed in Additional file 1: Table S3.

Das et al. BMC Genomics 2012 13:394   doi:10.1186/1471-2164-13-394
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