Additional file 4.

Table S3. Oligonucleotides employed in this study. This table lists the oligonucleotides utilized in this work. Section A shows the oligonucleotides used for DNA sequencing with the Sanger method, including those for the confirmation of the deletion that occurred in the PB12 strain (Figures 2, 3 and 4), the reported mutations provided by RN and WG (Table 1 and Tables S1 and S2 in Additional file 1 and Additional file 2) and the ones employed for gene disruption confirmation. Section B lists the oligos utilized for gene disruption with the Datsenko-Wanner methodology [46]. Section C lists the oligonucleotides utilized for RT-qPCR analysis not previously reported. The sequences of the oligos utilized for the remaining genes listed in Table 3, have been previously published [9-11] (see Materials and Methods).

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Aguilar et al. BMC Genomics 2012 13:385   doi:10.1186/1471-2164-13-385