Northern blot analysis of accumulation of sRNAs in plant organs. RNA from tissues of fresh lettuce leaf, green tomato fruit, mature soybean seed, soybean leaf, mature corn seed, corn leaf, corn root, dehulled rice grain (Oryza sativa spp. japonica cv. Nipponbare) was probed for corn miR168 sequence (Panel A); soybean miR168 sequence (Panel B), or miR166 (Panel C). Twenty-one base synthetic RNA oligonucleotides were included on the gel at various concentrations (12.5-400 pg) for semi-quantitative comparison.
Zhang et al. BMC Genomics 2012 13:381 doi:10.1186/1471-2164-13-381