Table 2

Association of mean root growth in transgenic lines with pleiotropic resistance to two pests in two different greenhouse assays and insect herbivory in field tests
A. Line::gene SCN infested Root mass (g) Significant differences Range (g) n SCN FI (%)
X5 No 1.05 a 0.81–1.44 15 0 ± 0.0
Westag97 No nd nd 5 0 ± 0.0
X5 Yes 0.98 a 0.73–1.31 15 100 ± 13
X5::RLK No 0.64 bc 0.57–0.74 15 0 ± 0.0
X5::RLK Yes 0.38 c 0.26–0.49 15 60 ± 11
X5::RLK::Rhg4 Yes nd nd 2 11 ± 3
X5::RLK::rhg4 Yes nd nd 3 38 ± 6
Westag97 Yes nd nd 5 130 ± 11
Westag97::RLK Yes nd nd 5 12 ± 3
B. Line::gene Fungus infested Root mass (g) Significant differences Range (g) n Root RS Leaf DS
X5 No 7.80 a 4.90–8.78 15 1.0 ± 0.1 3.2 ± 0.7
X5 Yes 3.86 bc 3.08–4.80 15 4.5 ± 0.9 4.3 ± 0.8
X5::RLK No 6.10 a 4.77–7.81 15 1.0 ± 0.1 1.0 ± 0.3
X5::RLK Yes 4.64 b 4.00–6.22 15 1.5 ± 0.5 1.5 ± 0.4
Westag97 Yes 6.15 A 3.02–9.55 6 6.5 ± 1.9 6.3 ± 1.3
Westag97::RLK Yes 12.2 B 3.55–24.0 6 0.5 ± 0.3 1.8 ± 0.5
C. Line::gene Insect PI (%) Mean shoot dry weight (g) n Leaf defoliation (%) Seed No weight
X5 22 + 13 47.2 a 4 33 ± 4.0 40 ± 9 11.6 ± 0.3
X5::RLK 59 + 15 18.8 b 4 90 ± 5.0 36 ± 8 11.2 ± 0.3

Part A shows SCN female index in greenhouse grown seedlings at 28 days after SCN infestations. Pots were watered daily with 100 ml. Female index (FI) was the mean percentage of cysts of Hg Type 0 found on five plants per repetition compared to a susceptible genotype Essex. Part B shows the effects of the transgene on resistance to F. virguliforme. in greenhouse grown seedlings at 28 days after infestations. Pots were saturated with water to the 5 cm level. Leaf scorch was recorded as the mean disease severity (DS) measured on a 1–9 scale found on five plants per experimental repeat. Root rot severity (IS) was measured on a 1–5 scale. Panel C shows the percent insect incidence, defoliation by herbivorous insects and the consequent loss of biomass at harvest as mean dry weight per plant for field grown plants with 4 replications across 2 years.

Srour et al.

Srour et al. BMC Genomics 2012 13:368   doi:10.1186/1471-2164-13-368

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