Validation of the RNA-seq results with quantitative RT-PCR. The extent of mRNA association with polysomes was compared between mosquitoes fed on P. falciparum-infected and uninfected blood. Changes in the relative PS loading (PL) obtained by RNA-seq analysis were plotted against the corresponding changes obtained with real-time RT-PCR. For both RNA-seq and RT-PCR analysis, the mean values of PL were calculated based on data from three independent experiments. Sixteen genes were analyzed. The Pearson correlation coefficient (p =0.9280) and the best-fit linear-regression analysis (R2 = 0.8611) demonstrated a high degree of correlation of the magnitude of regulation between the two assays.
Mead et al. BMC Genomics 2012 13:366 doi:10.1186/1471-2164-13-366