Isolation of mRNA associated with polysomes. Midguts were collected from female adult An. gambiae mosquitoes at about 24 h after ingestion of P. falciparum-infected or uninfected blood. Cellular extracts were sedimented by centrifugation in a 10-60% sucrose gradient, and 15 fractions were collected. Absorbance profile at 254 nm (top) is shown for a representative sample together with analysis of rRNA extracted from each fraction. RNA was separated on 1% agarose/formaldehyde gel followed by SYBR gold staining. The position of the 80 S peak is indicated.
Mead et al. BMC Genomics 2012 13:366 doi:10.1186/1471-2164-13-366