Quantitative real-time PCR confirmation of genes expressed at different levels in mid intestine (a), liver (b) and skeletal muscle (c) of fish fed the PP diet compared to fish fed the MP diet for 6 genes identified by microarray analysis. Bars represent mean ± standard error of five fish, asterisks indicate significant (p < 0.05) differences. The genes chosen for the intestine were: Fatty acid-binding protein (Fabp2), Caspase-14 precursor, TCR alpha, IgM heavy chain, Tropomyosin-1 alpha chain (Tpm1) and Replication factor C subunit 3 (RFC3). The genes selected for the liver were: Serine protease HTRA1, TGF beta receptor, 28 S Ribosomal protein S18a (MRPS18A), T-cell immunoglobulin and mucin domain (Timd2), hepcidin I and TRC alpha. The gene chosen for the skeletal muscle were: cell death inducing protein (CDIP), acta1 protein, Transforming growth factor beta-1 (TGF beta 1), MHC class Ib antigen (RT1-CE5), 60Sribosomal protein L6 (60 S rib prot) and Heat shock protein 30.
Tacchi et al. BMC Genomics 2012 13:363 doi:10.1186/1471-2164-13-363