Vital and fixative stainings of the Δ9 desaturase mutants. (A) Representative images of the Nile Red-stained worms (upper rows for each strain) and of the Oil Red-O (lower rows) for the mutant and wild type strains. In each image, anterior is positioned to the left. Scale bar is 50 μm. (B) Quantification of the Nile-Red staining (n = 12 animals at least per genotype), of the Oil Red-O (n = 20 animals at least per genotype), of the global fatty acid content as measured by GC-FID (n = 8 replicates at least per genotype) and triglyceride/phospholipid measurements as obtained by LC-MS (n = 8 animals at least per genotype). The GC-FID data were normalised to the same number of worms in addition to the added internal standard (in the first column) and normalised for the internal standard and the total mg of protein, as measured by the Bradford assay for protein content (subsequent column). Data are reported as mean ± standard error of the mean (SEM).
Castro et al. BMC Genomics 2012 13:36 doi:10.1186/1471-2164-13-36