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A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in Caenorhabditis elegans

Cecilia Castro12, Funda Sar13, W Robert Shaw13, Masanori Mishima13, Eric A Miska13 and Julian L Griffin124*

Author Affiliations

1 Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK

2 Cambridge Systems Biology Centre, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK

3 Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK

4 The Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, Fulborn Road, Cambridge, CB1 9NL, UK

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BMC Genomics 2012, 13:36  doi:10.1186/1471-2164-13-36

Published: 20 January 2012

Additional files

Additional file 1:

Supplemental Material. 1. Figure S1 2. Table S1: List of the resonances assigned in the NMR spectra of C. elegans. 3. Table S2. List of the amino acids identified by the EZ:faast Free (Physiological) Amino Acid kit in C. elegans. 4. Table S3. Characteristics of the PLS-DA models obtained considering each strains against the wild type in the GC-MS experiments using the EZ:faast Free (Pysiological) Amino Acid kit. 5. Table S4. List of the most significant amino acids changes considering each mutant against the wild type. 6. Table S5. List of the fatty acids identified by the GC-FID analysis of C. elegans. 7. Table S6. Characteristics of the PLS-DA models obtained considering each strains against the wild type in the GC-MS experiments of the lipid fraction. 8. Table S7. List of the most significant fatty acid changes considering each mutant against the wild type. 9. Table S8. Characteristics of each PLS-DA models obtained considering each strains against the wild type in the LC-MS experiments of the lipid fraction.

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