Over-expression of miR-146a.A) Deep sequencing reads mapped to the miR-146a pre-miR sequence. The most frequent isomiR (red) of miR-146a expressed exogenously following transfection with pSM30-146a (transfected cells and EVs) is represented by a similar percentage of reads as for endogenous miR-146a. B) RT-qPCR quantification of miR-146a in non-transfected and pSM30-146a transfected HEK293T cells.
Guduric-Fuchs et al. BMC Genomics 2012 13:357 doi:10.1186/1471-2164-13-357