Relationship of reference sequence quality scores of putative SNPs and variant frequency (VF). High correlation between reference sequence quality scores of putative SNPs and VF was observed in the data set of all putative SNPs (A). When putative SNPs with a Sanger quality score of less than 35 were removed, the correlation disappeared, showing that SNPs with high VF are false-positive SNPs due to sequencing errors in BES Sanger sequences (B).
You et al. BMC Genomics 2012 13:354 doi:10.1186/1471-2164-13-354