Open Access Highly Accessed Research article

Comprehensive microRNA profiling in B-cells of human centenarians by massively parallel sequencing

Saurabh Gombar1, Hwa J Jung2, Feng Dong2, Brent Calder2, Gil Atzmon2,3,4, Nir Barzilai2,3,4, Xiao-Li Tian5, Joris Pothof6, Jan HJ Hoeijmakers6, Judith Campisi7, Jan Vijg2,3 and Yousin Suh2,3,4,8*

1 Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA

2 Department of Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA

3 Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA

4 Institute for Aging Research, Diabetes Research and Training Center, Albert Einstein College of Medicine, Bronx, NY, 10461, USA

5 Department of Human Population Genetics, Peking University, Beijing 100871, China

6 Department of Genetics, Erasmus Medical Center, Rotterdam, The Netherlands

7 Buck Institute for Research on Aging, Novato, CA 94945, USA

8 Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China

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BMC Genomics 2012, 13:353 doi:10.1186/1471-2164-13-353

Published: 31 July 2012

Additional files

Additional file 1:

Table S1. Summary of read/sequence counts for miRNA analysis from lymphoblastoid cell lines from centenarians (n = 3) and controls (n = 3).

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Additional file 2:

Table S2. Complete list of known (miRBase v 9) average miRNA read counts from lymphoblastoid cells from centenarians (n = 3) and controls (n = 3). All reads present in 50% or more samples with a read number greater than 10.

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Additional file 3:

Table S3. Sequencing identified isomiRs from B-Cells of Centenarians and Controls.

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Additional file 4:

Table S4. Putative and Confirmed Targets of differentially expressed miRNAs.

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Additional file 5:

Table S5. Significantly over-represented GO categories of predicted targets of miRNA differentially expressed in B-Cells of Centenarians as compared to Controls.

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