Open Access Highly Accessed Research article

A complete mitochondrial genome sequence of Ogura-type male-sterile cytoplasm and its comparative analysis with that of normal cytoplasm in radish (Raphanus sativus L.)

Yoshiyuki Tanaka, Mizue Tsuda, Keita Yasumoto, Hiroshi Yamagishi and Toru Terachi*

Author Affiliations

31 Laboratory, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, Kyoto, 603-8555, Japan

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BMC Genomics 2012, 13:352  doi:10.1186/1471-2164-13-352

Published: 31 July 2012

Additional files

Additional file 1:

List of repeated sequences in normal-type genome.Repeated sequences(>80 bp, >90% homology) in normal-type genome were listed.

Format: XLSX Size: 12KB Download file

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Additional file 2:

List of repeated sequences in Ogura-type genome.Repeated sequences(>80 bp, >90% homology) in Ogura-type genome were listed.

Format: XLSX Size: 23KB Download file

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Additional file 3:

The sequences homologous to Brassicaceae mitochondrial genome plotted on Ogura-type specific region I -III. A black line indicates the region specific to the Ogura-type mitochondrial genome. Black boxes indicate the sequence homologous to radish and/or other Brassicaceae mitochondrial genomes.

Format: PPT Size: 213KB Download file

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Additional file 4:

Validation of contig linkage by PCR analysis. The primer information used for this PCR analysis is described in Additional file 5.

Format: PPTX Size: 433KB Download file

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Additional file 5:

The primers used for the PCR analysis to validate the linkage between contigs of normal-type genome. Information on the primers used for the PCR analysis in Additional file 4.

Format: XLSX Size: 14KB Download file

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Additional file 6:

The primers used for PCR analysis to validate the short repeats. Information on the primers used for PCR and sequencing, in order to confirm short repeats in Ogura-type genome.

Format: XLSX Size: 11KB Download file

Open Data