Additional file 7.Figure S2. A wild type copy of the VNG0258H gene supplied on a plasmid (pMTFcmyc vector, [1]) complements the ΔVNG0258H growth defects. (A) Box-whisker plots depicting growth rates of H. salinarum strains in the bioscreen C (Δura3 parent, ΔVNG0258H mutant, and ΔVNG0258H mutant complemented in trans) during the 12 hours following H_{2}O_{2} shock (mid-logarithmic phase addition of H_{2}O_{2}). Horizontal lines within each box represent the median growth rate across 24 replicate trials (8 biological replicates, 3 technical replicates) for each strain in each condition. Boxes represent the interquartile range (IQR), and whiskers are minimum and maximum values within 1.5 times the IQR. Concentrations of H_{2}O_{2} added are indicated on the X-axis, whereas the Y-axis quantifies growth rate. (B) Box-whisker plot depicting lag phase addition of H_{2}O_{2} to Bioscreen cultures. Boxes, median lines, and whiskers are as in (A). Y-axis expresses the growth rate of the ΔVNG0258H or trans-complemented strains as a function of Δura3 growth rate. (C) Box-whisker plot depicting survival ratios 24 hours after mid-logarithmic phase addition of 25 mM H_{2}O_{2} to batch cultures. (D) Box-whisker plot depicting growth rates following mid-logarithmic phase addition of PQ to batch cultures. Growth rates are expressed as a function of Δura3 parent strain growth. Format: PDF Size: 773KB Download file This file can be viewed with: Adobe Acrobat Reader Sharma et al. BMC Genomics 2012 13:351 doi:10.1186/1471-2164-13-351 |