Additional file 7.

Figure S2. A wild type copy of the VNG0258H gene supplied on a plasmid (pMTFcmyc vector, [1]) complements the ΔVNG0258H growth defects. (A) Box-whisker plots depicting growth rates of H. salinarum strains in the bioscreen C (Δura3 parent, ΔVNG0258H mutant, and ΔVNG0258H mutant complemented in trans) during the 12 hours following H2O2 shock (mid-logarithmic phase addition of H2O2). Horizontal lines within each box represent the median growth rate across 24 replicate trials (8 biological replicates, 3 technical replicates) for each strain in each condition. Boxes represent the interquartile range (IQR), and whiskers are minimum and maximum values within 1.5 times the IQR. Concentrations of H2O2 added are indicated on the X-axis, whereas the Y-axis quantifies growth rate. (B) Box-whisker plot depicting lag phase addition of H2O2 to Bioscreen cultures. Boxes, median lines, and whiskers are as in (A). Y-axis expresses the growth rate of the ΔVNG0258H or trans-complemented strains as a function of Δura3 growth rate. (C) Box-whisker plot depicting survival ratios 24 hours after mid-logarithmic phase addition of 25 mM H2O2 to batch cultures. (D) Box-whisker plot depicting growth rates following mid-logarithmic phase addition of PQ to batch cultures. Growth rates are expressed as a function of Δura3 parent strain growth.

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Sharma et al. BMC Genomics 2012 13:351   doi:10.1186/1471-2164-13-351