qRT-PCR was used to validate microarray results for six genes. Microarray and qRT-PCR showed similar patterns for five of the six genes: glial fibrillary acidic protein (gfap), proopiomelanocortin a (pomca), selenoprotein P1a (sepp1a), synaptoporin (synpr), and deiodinase, iodothyronine, type II (dio2). Validation failed for glutamate decarboxylase 2 (gad2), suggesting that additional factors may be affecting hybridization to the microarray. Abbreviations: G = Gaighatta, N = Nadia, S = Scientific Hatcheries, T = TM1.
Drew et al. BMC Genomics 2012 13:323 doi:10.1186/1471-2164-13-323