Additional file 2:.
Annotation results of 4565 differentially expressed genes. Transcription level of each gene was deduced by determining the total number of reads mapped to each gene using Picard tools. Differentially expressed genes were identified by the DESeq package in R software using two-fold change (log2 (fold-change) ≥1 or ≤-1) and p-value <0.05 (cut-off at 5% false discovery rate (FDR)) as the threshold. “baseMean”, the base mean of counts divided by the size factors. “baseMean A”, the base mean for the counts of PBS-mock condition. “baseMean B”, the base mean for the counts of WED-immunized condition. “rpkm_avg_c”, the expression level of PBS-mock group. “rpkm_avg_v”, the expression level of WED-immunized group. “ZF-Annotation”, annotation of the differentially expressed genes through BLASTN similarity searches against the Ensembl zebrafish RefSeq mRNA database.
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Yang et al. BMC Genomics 2012 13:319 doi:10.1186/1471-2164-13-319