qPCR analysis of the genes related to MHC-II processing pathway in zebrafish following WED-immunization during the first five days. Lysosomal membrane glycoprotein 2 (lamp2), MHC class II DAB gene (MHC-II dab), CD74 molecule (cd74) and MHC class II, transactivator (CIITA) were validated. qPCR analysis for the expressions of the above immune-related genes in livers (gray bars) and spleens (white bars) of zebrafish vaccinated with WED. The experimental procedure was the same as in the legend of Figure 5.
Yang et al. BMC Genomics 2012 13:319 doi:10.1186/1471-2164-13-319