Dual-luciferase reporter assay for validation of the 3′ UTR ofGHRas the target site of the let-7bin vitrocell system. DF-1 cells were transfected with pmirGLO-let-7b-GHR 3′ UTR, pmirGLO-let-7b-GHR 3′ UTR mutation plasmid, or the control plasmid. The activity of firefly luciferase (luc2) and Renilla luciferase (hRluc-neo) was measured and relative values (luc2/hRluc-neo) calculated.
Lin et al. BMC Genomics 2012 13:306 doi:10.1186/1471-2164-13-306