Effectiveness of normalization of the cDNA library using duplex-specific nuclease. (A) Agarose gel smears show that non-normalized cDNA (treated with water) has distinct bands representing highly expressed genes, but these bands are absent in the normalized sample treated with duplex-specific nuclease (DSN). (B) RT-PCR analysis of transcript levels indicates that representation of the highly expressed genes Pl-β-actin and Pl-α-tubulin in the library is decreased after normalization with DSN. Representation of three lowly expressed genes, Pl-wnt-1, Pl-otx-2, and Pl-hox-Z, is increased, consistent with successful normalization. Standard error bars are shown.
Nyberg et al. BMC Genomics 2012 13:287 doi:10.1186/1471-2164-13-287