Open Access Highly Accessed Research article

Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA

Anastasios Mastrokolias1, Johan T den Dunnen12, GertJan B van Ommen1, Peter AC 't Hoen1 and Willeke MC van Roon-Mom1*

Author Affiliations

1 Center for Human and Clinical Genetics, Leiden University Medical Center, Einthovenweg 20, 2333ZC, Leiden, The Netherlands

2 Leiden Genome Technology Center, Leiden University Medical Center, Einthovenweg 20, 2333ZC Leiden, The Netherlands

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BMC Genomics 2012, 13:28  doi:10.1186/1471-2164-13-28

Published: 18 January 2012

Additional files

Additional file 1:

Hemoglobin qPCR results. qPCR results for the hemoglobins before and after globin reduction. 4 non reduced and 4 reduced samples were loaded in triplicates and beta actin (ACTB) was used as a reference gene.

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Additional file 2:

Correlation matrix for the Globin reduced samples. Upper panels represent correlation values of log transformed data with stars depicting significance. Lower panels contain scatter plots of log transformed globin reduced samples.

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Additional file 3:

List of 87 down-regulated genes. List of 87 genes detected at significantly lower levels in the globin reduced samples.

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Additional file 4:

qPCR primer Sequences. Primer sequences used for qPCR validation of HBA, HBB transcript levels.

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