Table 5

Completeness of transcripts and sequence conservation for some proteins involved in cellular spatial organization
Query protein (GenBank Accession Number) Amino acids in query sequence Amino acids of query covered by best BLAST hit(s) % amino-acid sequence identity (number of gaps) Length ofAiptasiacontig (bp) Positions in contig covered by best BLAST hit
Mouse Cdc42 (P60766) 191 1-191 92 (0) 1,399 275-847
Mouse cyto-plasmic actin 1 (P60710) 375 2-375 97 (0) 1,455 117-1,247
Mouse tubulin α1B (P05213) 451 1-432 97 (0) 1,971 1,879-584
Mouse tubulin β5 (P99024) 444 1-427 98 (0) 2,592 1,529-249
Mouse septin-2 (P42208) a 361 139-329 65 (14b) 1,979 3-617
Mouse kinesin 1 heavy chain (Q61768) 963 3-199 72 (0) 906 592-2
453-947 53 (14) 2,138 6–1,514
Mouse myosin 8 (P13542) 1,937 23-1,903 50 (14) 7,798 7,650-2,032
Mouse dynein heavy chain 1 (Q9JHU4) 4,644 21-216 53 (4) 790 191-790
836-4,643 73 (30) 11,600 3–11,414

a Our unpublished studies have shown that as expected, the A. pallida genome encodes multiple septins and that, like other septins [42], these contain the three conserved motifs of a GTP-binding site in their N-terminal regions. We do not yet know why none of these transcripts appears in full-length form in the current transcriptome.

b The predicted A. pallida protein has a single insertion of 14 amino acids near the C-terminus of the “septin-unique element” [42].

Lehnert et al.

Lehnert et al. BMC Genomics 2012 13:271   doi:10.1186/1471-2164-13-271

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