Additional file 4.

Percentage methylated cytosines in bisulfite-sequencing targets in relationship to RPKM and maximum per-nucleotide MeDIP-seq coverage from genomic data. Average percentage methylated cytosines was calculated for eight targets PCR-amplified from three bisulfite-treated bud types. A. Percentage methylated cytosines plotted against RPKM calculated for each target region. B. Percentage methylated cytosines plotted against maximum per-nucleotide coverage within target region.

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Vining et al. BMC Genomics 2012 13:27   doi:10.1186/1471-2164-13-27