Open Access Research article

Postmortem cardiac tissue maintains gene expression profile even after late harvesting

Simone Gupta1, Marc K Halushka2, Gina M Hilton1 and Dan E Arking1*

  • * Corresponding author: Dan E Arking arking@jhmi.edu

  • † Equal contributors

Author Affiliations

1 McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

2 Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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BMC Genomics 2012, 13:26  doi:10.1186/1471-2164-13-26

Published: 17 January 2012

Additional files

Additional file 1:

Figure S1: RIN range in "warm- and "cold-24 hour autolysis" samples. The range of the RIN values in the warm-24 autolysis samples was 2.7 - 8.6, while the range in the cold- 24 autolysis samples was 3.5 - 8.6. The means of the RIN values in the "warm" and "cold" 24 hour autolysis samples were 5.1 (SD 1.53) and 6.9 (SD 1.45), respectively, and this difference was not statistically significant (P = 0.11).

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Additional file 2:

Table S1: Postmortem fluctuating genes common in autopsy and explant hearts. The gene symbol and description of the 21 genes, which showed variation in gene expression during 24 hours of autolysis in both the autopsy and explant hearts.

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Additional file 3:

Table S2: List of 374 upregulated (Table S2a)and 108 downregulated (Table S2b) genes in the explant/failed heart. List of ~480 genes differentially up- and down-regulated between the explant and autopsy hearts. Among these differentially expressed genes, 374 genes were upregulated and 108 genes were downregulated in the explant/failed heart.

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