Table 2

Noteworthy genes or loci absent in theM. ulceransMRCA
Gene(s) Locus_tag(s) Comments Reference
selA MMAR_5190 selenocysteine synthase, required for the synthesis of proteins containing selenocysteine. [1]
MMAR_5194 MMAR_5195 Formate dehydrogenase alpha and beta subunits, selenocysteine-containing. Likely role in anaerobic growth. [1]
MMAR_2615 Anaerobic dehydrogenase (putative nitrate reductase), possible role in anaerobic growth.
lgt MMAR_2416 Lgt acylates prolipoproteins, thus key role in lipoprotein synthesis. Lgt mutant of S. aureus is less immunogenic. [45-47]
plcB_2, MMAR_1485 M. marinum has six plc genes. Four of these are absent in all M. ulcerans. Phospholipase C enzymes can cause direct or indirect enzymatic hydrolysis of host cell membrane phospholipids and appear important for mycobacterial intracellular survival. [48]
plcB_5, MMAR_3656
plcB_6, MMAR_4722
plcB_3 MMAR_0284
cstA MMAR_1616 Carbon starvation protein, CstA. In E. coli, cstA encodes a peptide transporter and is induced by carbon starvation. Maybe part of a redundant stress response system in M. ulcerans. [49]
cueO MMAR_1618 Multicopper oxidases protect against oxidative stress. This enzyme has functions in tolerance to copper, and iron and manganese oxidation in a range of bacteria. It catalyses the oxidation of cuprous copper, ferrous iron and diphenolic compounds. In Salmonella, a cueO deletion mutant is less virulent in mouse model. Divergent transcriptional arrangement with cstA. [50]
idsB1, MMAR_3212 Catalyzes the trans-addition of three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate which is a precursor of the ether-linked lipids. Impact here of diverting all isoprenoid biosynthesis to the non-mevalonate pathway is not known. Possibly more favourable energetically if spending cellular resources on mycolactone synthesis. [1]
idsB2, MAR_3219
idsA1 MMAR_5095

Doig et al.

Doig et al. BMC Genomics 2012 13:258   doi:10.1186/1471-2164-13-258

Open Data