Table 1

Effect of stress on haematological variables1in control and weaned calves
Days post weaning P-values
Variable 0 1 2 3 7 T S T × S
Total Leukocytes C 13.3 ± 0.97 13.8 ± 1.44 13.9 ± 0.71 15.6bx ± 1.08 13.9x ± 0.61 NS NS *
(×103 cells/μL) W 12.2 ± 0.91 13.2 ± 1.34 12.1 ± 0.66 12.2y ± 1.01 11.3y ± 0.57
Neutrophils, % C 23.7 ± 2.63 24.9x ± 2.30 28.9 ± 2.92 34.9b ± 3.12 28.9 ± 2.29 NS NS ***
W 30.9 ± 2.45 43.4cy ± 2.14 29.7 ± 2.72 30.1 ± 2.90 26.3 ± 2.14
Lymphocytes, % C 63.7 ± 2.87 63.9x ± 2.17 58.3 ± 2.44 53.3b ± 2.59 59.0 ± 2.50 NS NS ***
W 58.4 ± 2.67 44.9cy ± 2.02 58.8 ± 2.27 57.8 ± 2.41 64.1 ± 2.33
N:L Ratio C 0.38 ± 0.076 0.41x ± 0.061 0.52 ± 0.079 0.69b ± 0.098 0.51 ± 0.056 NS NS ***
W 0.57 ± 0.071 0.99cy ± 0.057 0.52 ± 0.074 0.54 ± 0.091 0.42 ± 0.052
Eosinophils, % C 2.7 ± 0.46 1.9a ± 0.23 3.4 ± 0.86 3.7 ± 0.89 3.5 ± 1.18 NS NS NS
W 1.6 ± 0.43 1.9 ± 0.21 2.3 ± 0.79 1.9 ± 0.83 1.8 ± 1.09
Monocytes, % C 6.6 ± 0.36 7.1 ± 0.44 7.2 ± 0.39 6.1 ± 0.63 5.9 ± 0.45 NS NS *
W 7.5 ± 0.33 6.9 ± 0.41 6.9 ± 0.36 7.5 ± 0.59 5.7b ± 0.42
RBC C 11.9 ± 0.21 11.8 ± 0.19 12.3b ± 0.24 12.1 ± 0.27 12.1 ± 0.28 NS NS NS
(×106 cells/μL) W 11.6 ± 0.22 11.6 ± 0.21 11.8 ± 0.26 11.8 ± 0.29 11.8 ± 0.29
HCT, % C 34.6 ± 0.68 34.4 ± 0.69 35.9b ± 0.84 35.5 ± 0.85 35.3 ± 0.81 NS * NS
W 33.5 ± 0.64 33.4 ± 0.65 34.1 ± 0.78 34.5 ± 0.79 34.2 ± 0.76
HGB (g/dL) C 13.0 ± 0.19 13.1 ± 0.25 13.5b ± 0.28 13.4 ± 0.29 13.4 ± 0.29 NS * NS
W 12.7 ± 0.19 12.8 ± 0.23 12.9 ± 0.26 12.9 ± 0.28 12.9 ± 0.27
MCHC (g/dL) C 37.7 ± 0.28 38.1b ± 0.24 37.7 ± 0.22 37.7 ± 0.23 37.9 ± 0.29 NS NS *
W 37.9 ± 0.26 38.5c ± 0.22 37.8 ±0.21 37.5a ± 0.21 37.7 ± 0.27
Platelet number C 640.1 ± 63.06 697.2x ± 60.73 700.4 ± 55.59 684.8 ± 57.03 665.2 ± 49.91 NS * NS
(×103 cells/μL) W 604.5 ± 58.79 469.4ay ± 56.62 596.4 ± 51.83 562.3 ± 53.17 536.3 ± 46.54

1Haematological variables; total leukocyte, neutrophil and lymphocyte percentage, neutrophil:lymphocyte (N:L) ratio, eosinophil and monocyte percentage, red blood cell (RBC) number, haematocrit (HCT) percentage, haemoglobin (HGB) and mean corpuscular haemoglobin concentration (MCHC). The values are expressed as least squares means (Lsmeans) ± s.e.

C = Control, W = Weaned, T = treatment, S = sampling time, T × S = treatment × sampling time interaction, NS = not significant (P > 0.05).

* = P < 0.05, ** = P < 0.01, *** = P < 0.001.

a,b,cWithin rows, Lsmeans differ from pre-weaning baseline by P < 0.05, P < 0.01 and P < 0.001, respectively.

x,yBetween rows, Lsmeans differ between treatments by P < 0.05.

O’Loughlin et al.

O’Loughlin et al. BMC Genomics 2012 13:250   doi:10.1186/1471-2164-13-250

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