Figure 2 .
Bioinformatic pipeline employed in the analysis of RNA-seq data. Following sequencing on the GAII, FastQC was used to assess sequencing quality prior to aligning reads to the bovine genome with Bowtie. Counts were then assigned to each gene/exon with HTseq prior to the calculation of differential gene expression with EdgeR.
O’Loughlin et al. BMC Genomics 2012 13:250 doi:10.1186/1471-2164-13-250