Table 2

Protein identification of 30 spots with highest Mascot scores picked on deep purple stained 2D-gel
Spot Nb Fold changes Vectorbase access number Mascot score Top ranking match Predicted PI Predicted mass (kDa) % sequence coverage Species
Proteases
2 1.88 AAEL015386_a 249 dipeptidyl-peptidase 4.91 84.9 42 Ae. aegypti
3 2.77 AAEL015386_b 149 dipeptidyl-peptidase 4.91 84.9 22 Ae. aegypti
4 + 1.81 AAEL012774_a 237 protease m1 zinc metalloprotease 4.81 102.5 41 Ae. aegypti
5 + 2.34 AAEL012774_b 162 protease m1 zinc metalloprotease 4.81 86.7 42 Ae. aegypti
20 + 1.81 AAEL012776_a 135 protease m1 zinc metalloprotease 5.19 103.3 30 Ae. aegypti
21 2.74 AAEL012776_b 146 protease m1 zinc metalloprotease 5.19 103.3 27 Ae. aegypti
19 3.81 AAEL012776_c 136 protease m1 zinc metalloprotease 5.19 103.3 22 Ae. aegypti
Detoxification enzymes
45 + 3.01 CPIJ019700 119 cytochrome P450 7.6 58.4 41 C. quinquefasciatus
Kinases-Phosphatases
22 1.58 AAEL003313 62 alkaline phosphatase 5.46 61.0 23 Ae. aegypti
24 1.92 AAEL003298_a 186 alkaline phosphatase 5.28 58.8 39 Ae. aegypti
25 1.16 AAEL003298_b 194 alkaline phosphatase 5.23 58.3 39 Ae. aegypti
Other enzymes
6 + 1.78 AAEL010532 146 alpha-amylase 4.82 68.9 37 Ae. aegypti
13 1.27 AAEL004580 129 beta-galactosidase 4.87 74.1 34 Ae. aegypti
11 2.06 AAEL002827_a 61 ATP synthase beta subunit 5.03 53.9 32 Ae. aegypti
23 2.29 AAEL002827_b 152 ATP synthase beta subunit 5.03 53.9 55 Ae. aegypti
16 2.06 AAEL008787_a 162 V-ATP synthase subunit alpha 5.26 68.5 31 Ae. aegypti
17 2.19 AAEL008787_b 231 V-ATP synthase subunit alpha 5.26 68.5 42 Ae. aegypti
18 1.82 AAEL008787_c 132 V-ATP synthase subunit alpha 5.26 68.5 30 Ae. aegypti
27 1.51 AAEL005798_a 200 V-ATP synthase subunit beta 5.31 54.8 49 Ae. aegypti
28 1.69 AAEL005798_b 177 V-ATP synthase subunit beta 5.31 54.8 44 Ae. aegypti
29 1.72 AAEL005798_c 187 V-ATP synthase subunit beta 5.31 55.4 53 Ae. aegypti
30 2.06 AAEL005798_d 229 V-ATP synthase subunit beta 5.38 55.5 52 Ae. aegypti
31 2.39 AAEL005798_e 197 V-ATP synthase subunit beta 5.38 55.4 48 Ae. aegypti
32 1.89 AAEL005798_f 181 V-ATP synthase subunit beta 5.38 55.4 56 Ae. aegypti
52 2.14 AAEL012035_a 93 V-ATP synthase subunit E 5.91 25.7 35 Ae. aegypti
53 2.21 AAEL012035_b 88 V-ATP synthase subunit E 5.91 25.7 38 Ae. aegypti
Other functions
8 + 10.74 AAEL001005_a 195 calreticulin 4.42 47.0 43 Ae. aegypti
8 2.56 AAEL001005_a 226 calreticulin 4.42 47.0 49 Ae. aegypti
9 + 3.14 AAEL001005_b 210 calreticulin 4.42 47.0 49 Ae. aegypti
10 + 2.75 AAEL001005_c 158 calreticulin 4.42 47.0 48 Ae. aegypti
12 + 3.01 AAEL001005_d 93 calreticulin 4.42 46.7 35 Ae. aegypti

Proteins are classified according to their putative function using the same 13 categories as for transcriptomic data. When different spots pointed to the same protein, they were differentiated using letters after the access number. For each identification, the predicted pI, the predicted mass in kiloDaltons (kDa), the percentage of sequence coverage and the species and database matched are indicated.

Tetreau et al.

Tetreau et al. BMC Genomics 2012 13:248   doi:10.1186/1471-2164-13-248

Open Data