2D-DIGE gel and corresponding picked silver stained gel. BBMV proteins were prepared from resistant and susceptible Aedes aegypti larval midguts and separated using 2D-DIGE. The spots appear in yellow when corresponding to proteins present at approximately equal amounts in both resistant and susceptible BBMV samples, green for those only present in the susceptible BBMV labeled with Cy3, and red for those only present in the resistant BBMV labeled with Cy5. The x-axis shows pI values from 4 to 7 and the y-axis shows apparent molecular weight in kilodaltons (kDa). Panel A. Overlay of Cy3 and Cy5, and Panel B. Gel co-run stained with deep purple. All the 56 spots picked either on the first, the second or both the two gels, corresponding to the two biological replicates, are noted on this gel.
Tetreau et al. BMC Genomics 2012 13:248 doi:10.1186/1471-2164-13-248