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Open Access Research article

Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis

Eva Apweiler1, Katrin Sameith1, Thanasis Margaritis1, Nathalie Brabers1, Loes van de Pasch1, Linda V Bakker12, Dik van Leenen1, Frank CP Holstege1 and Patrick Kemmeren1*

Author Affiliations

1 Molecular Cancer Research, University Medical Centre Utrecht, Universiteitsweg 100, Utrecht, the Netherlands

2 Netherlands Bioinformatics Centre, Geert Grooteplein 28, 6525, GA, Nijmegen, the Netherlands

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BMC Genomics 2012, 13:239  doi:10.1186/1471-2164-13-239

Published: 14 June 2012

Abstract

Background

Cellular glucose availability is crucial for the functioning of most biological processes. Our understanding of the glucose regulatory system has been greatly advanced by studying the model organism Saccharomyces cerevisiae, but many aspects of this system remain elusive. To understand the organisation of the glucose regulatory system, we analysed 91 deletion mutants of the different glucose signalling and metabolic pathways in Saccharomyces cerevisiae using DNA microarrays.

Results

In general, the mutations do not induce pathway-specific transcriptional responses. Instead, one main transcriptional response is discerned, which varies in direction to mimic either a high or a low glucose response. Detailed analysis uncovers established and new relationships within and between individual pathways and their members. In contrast to signalling components, metabolic components of the glucose regulatory system are transcriptionally more frequently affected. A new network approach is applied that exposes the hierarchical organisation of the glucose regulatory system.

Conclusions

The tight interconnection between the different pathways of the glucose regulatory system is reflected by the main transcriptional response observed. Tps2 and Tsl1, two enzymes involved in the biosynthesis of the storage carbohydrate trehalose, are predicted to be the most downstream transcriptional components. Epistasis analysis of tps2Δ double mutants supports this prediction. Although based on transcriptional changes only, these results suggest that all changes in perceived glucose levels ultimately lead to a shift in trehalose biosynthesis.

Keywords:
Regulatory networks; Glucose signalling; Trehalose biosynthesis; Gene expression profiling; Saccharomyces cerevisiae